PCR laboratory purification project

PCR Cleanroom Gene Amplification Laboratory

PCR stands for Polymerase Chain Reaction. It is a molecular biology technique used to amplify specific DNA fragments, which can be seen as special DNA replication outside of the body. PCR laboratory is also known as "gene amplification laboratory" The floor plan design of the PCR laboratory is shown below:

According to the Interim Measures for the Management of Clinical Laboratory Amplification Testing Laboratories, the principles for setting up clinical gene amplification testing laboratory areas are as follows:

（1） Principles for Regional Setting of Clinical Gene Amplification Testing Laboratory

1. Reagent storage and preparation area

2. Specimen preparation area

3. Preparation of amplification reaction mixture and amplification zone

4. Amplification product analysis area

If a fully automated analyzer is used, the regions can be merged appropriately.

（2） Each work area must have clear markings to avoid mixing equipment and items in different work areas.

（3） Entering each work area must strictly follow a single direction, namely reagent storage and preparation area ->specimen preparation area ->amplification reaction mixture preparation and amplification area ->amplification product analysis area.

（4） Use different work clothes (such as different colors) in different work areas. Staff members are not allowed to take their work clothes out when leaving each work area.

The "Sixteen Character Formula" for Building a PCR Laboratory:

• Each district is independent

Pay attention to the wind direction

• Adapt to local conditions

• Convenient for work

The BIOBASE purification project strictly follows the relevant standards and specifications such as GB50591-2010 Cleanroom Construction and Acceptance Specification, GB50346-2011 Biosafety Laboratory Building Technical Specification, GB19489-2008 General Requirements for Laboratory Biosafety, and the Interim Measures for the Management of Clinical Laboratory Amplification Testing Laboratories. Professional design, construction, commissioning, and testing are carried out to complete the construction of the clean operating room with high-quality services.